Phenotypic Platform: ZebraSens

About

About

Co-Heads: Mireille Rossel and Benjamin Delprat

Steering Commitee: Anne-Françoise Roux, Mireille Rossel, Laurent Givalois, Tangui Maurice, Benjamin Delprat, Jean-Michel Verdier

The ZebraSens platform was created at the initiative of 2 laboratories: MMDN (JM Verdier) and LGMR (AF Roux) in order to analyze at the functional level the sensory and behavioral deficits in degenerative pathologies.

This platform brings together, on the same site and close to the breeding platform of the MMDN Laboratory "Ze-Neuro", state-of-the-art equipment allowing the analysis of vision, hearing and locomotor behavior of zebrafish at different stages, from larvae to adults.

The vision is evaluated by:

- A « Visiobox® », able to measure the OKR response (OptoKinetic Response test). This test assesses the visual acuity of zebrafish in a stress-free and automated environment.

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The visual acuity measured by OKR involves the movement of the eyes of the fish. This movement is generated by the scrolling of white and black lines at regular intervals and strongly contrasting. It is possible to reduce the size or contrast of black bands to increase the difficulty and accurately assess the visual acuity of a wild type or mutant fish. It is possible to measure the visual acuity of 4 fish at the same time.

- The « Zebrabox® » and VMR test (Visual Motor Response), allows to measure the locomotor response to light flashes, isolated or repeated.

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The audition is evaluated by:

- The « Zebrabox Fast® » containing a vibratory module. This system is able to measure the curvature of the fish following sound stimulation of chosen frequency and intensity. This curvature is characteristic of the avoidance behavior of a predator and provides information on the auditory acuity of the fish.

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The locomotor behavior is evaluated by:

- The « Zebrabox classique® »: The larvae are videotracked and it is thus possible to determine the distance traveled, the position, the stop phases and the activity of the larvae at different stages.

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- The « ZebraCube® »: The adult fish are videotracked and it is thus possible to determine the distance traveled, the position, the stop phases and the activity of the fish. In addition, it is possible to measure social behavior (shoaling) and spatial memory (T-maze and double H-maze).

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- The « Shuttle Box® »: This complementary device of the "ZebraCube" measures the active avoidance of adult fish following electrical stimuli.

Acknowledgments: The « ZebraSens Phenotying” platform was mainly financed by the SOS Rétinite France Association, through the Caisse d'Epargne.

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Team

Mireille Rossel, Scientific officer

rossel

Lecturer, EPHE

Contact : mireille.rossel(at)umontpellier.fr / mireille.rossel(at)ephe.sorbonne.fr

Phone : 04 67 14 38 15

Benjamin Delprat, Scientific officer

benjamin

Research fellow, Inserm

Contact : benjamin.delprat(at)inserm.fr

Phone : 04 67 14 36 23

References

  • Nasri A, Valverde AJ, Roche DB, Desrumaux C, Clair P, Beyrem H, Chaloin L, Ghysen A, Perrier V (2016) Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations. Int J Mol Sci. 17 :21-37.
  • Angebault C, et al. Recessive mutations in RTN4IP1 cause isolated and syndromic optic neuropathies. American Journal of Human Genetics, 2015, 97(5):754-60.
  • Huc-Brandt S, Hieu N, Imberdis T, Cubedo N, Silhol M, Leighton PLA et al. Zebrafish prion protein PrP2 controls collective migration process during lateral line sensory system development. PloS One, 2014; 9: e113331.
  • Gamba L, Cubedo N, Ghysen A, Lutfalla G, Dambly-Chaudiere C. Estrogen receptor ESR1 controls cell migration by repressing chemokine receptor CXCR4 in the zebrafish posterior lateral line system. Proc Natl Acad Sci U S A, 2010; 107: 6358358863
  • Cubedo N, Cerdan E, Sapede D, Rossel M. CXCR4 and CXCR7 cooperate during tangential migration of facial motoneurons. Molecular and Cellular Neuroscience, 2009, 40:474-484.
  • Sapede D, Rossel M, Dambly-Chaudiere C, Ghysen A. Role of SDF1 chimiokine in the development of lateral line efferent and facial motor neurons. Proc Natl Acad Sci U S A, 2005 ;102:1714-8.